Groups of mice of four different syngeneic strains were sensitized subcutaneously with 100 μg of BtE on days 0 and 7 and challenged four times intranasally, at days 8, 10, 12, and 14, with 10 μg of BtE. 072405/Z/03/Z). Hamelmann E, Tadeda K, Schwarze J, Vella AT, Irvin CG, Gelfand EW: Development of Eosinophilic Airway Inflammation and Airway Hyperresponsiveness Requires Interleukin-5 but Not Immunoglobulin E or B Lymphocytes. BtE-sensitized animals had more total serum IgE as well as higher titers of anti-BtE IgE antibodies than the saline control group (Figure 5D and 5E; p < 0.05; Student's t test and Mann-Whitney's test, respectively). Departamento de Biointeração, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Av. The species Blomia tropicalis is typically found only in tropical or subtropical regions. Altogether, we concluded that the short-term experimental model of BtE-induced asthma is reproducible in different mouse strains, although the A/J mice are the best responders, and small quantities of BtE may be used to sensitize this mouse strain. Thus, it appears that alum is required to achieve fully polarized Th2 responses to BtE. We sought to compare allergen inflammatory pulmonary response in previously sensitized mice orally administered with dust mites Blomia tropicalis (Bt) or Dermatophagoides pteronyssinus (Dp). Experimental data indicate that inbred mouse strains differ in their ability to mount an allergen-induced asthmatic response [17, 18]. Google Scholar. Int Arch Allergy Immunol 1997,113(1–3):102–4. [20]. Mice of all strains had lung inflammatory-cell infiltration and increased levels of anti-BtE IgE antibodies, but these responses were significantly more intense in A/J mice than in CBA/J, BALB/c or C57BL/6J mice. No differences in numbers of macrophage, lymphocyte and neutrophils in the BALF were observed among the mice of all four strains (p > 0.05, ANOVA; data not shown). The first was to study the anti-B. El este așa de mic, încât poate fi transportat de particulele de praf ce plutesc în aer sau de mici curenți de aer. This article is published under license to BioMed Central Ltd. Lowry OH, Rosenbrough NJ, Faar AL, Randall RJ: Protein measurement with the folin phenol reagent. Int Arch Allergy Immunol 2001,126(4):286–93. (A) Total cell count in the BALF. Unreviewed (50) TrEMBL. Mice from all four studied strains, sensitized and challenged with BtE had higher levels of BtE-specific IgE as revealed by PCA, than the alum- and saline-treated control mice (p < 0.001 for A/J, p < 0.01 for CBA/J, and p < 0.05 for C57Bl/6 and BALB/c, Tukey's test; Figure 1D). However, in humans, most cases of asthma are due to aeroallergens, and OVA-induced asthma is far from being a common event. Existem duas espécies mais prevalentes no Brasil - Dermatophagoides pteronyssinus e D. farinae.O ácaro Dermatophagoides é considerado o principal agente causador de alergia respiratória no Brasil junto com outro ácaro conhecido como Blomia tropicalis. J Clin Immunol 2004,24(5):533–41. IFN-γ and IL-10 concentrations in the BALF from BtE-sensitized or saline-treated mice of all tested mouse strains were low, and no statistically significant differences were found among the studied groups and their negative controls (data not shown). Rev Inst Med Trop Sao Paulo 2004,46(1):1–8. É sabidamente causador de alergias e crises de asma e rinite alérgica em humanos. Reitor Miguel Calmon, Sem n°. Its use as an assay for eosinophil differentiation factors. Another hypothesis is that proteases present in the BtE cleave CD23, a negative regulator of IgE production [34]. Eight to 10 week-old CBA/J, BALB/c, A/J and C57BL/6 male mice, and 3 to 4 month-old Wistar rats, were bred and maintained at the animal houses of the Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Salvador, Brazil, and of the Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil. Pulm Pharmacol 1989,2(2):59–74. Baqueiro, T., Russo, M., Silva, V.M. See more. Am J Respir Crit Care Med 1997,155(1):343–50. Control groups received only alum and were challenged with saline or with BtE. Mite allergens are considered one of the most potent allergens in house dust. (F and G) representative lung sections stained with hematoxylin and eosin of a saline-injected animal (F) and a Bt-sensitized animal (G). Finally, immunization with a relatively low BtE dose (10 μg per subcutaneous injection per mouse) was able to sensitize A/J mice, which were the best responders to high-dose BtE immunization, for the development of allergy-associated immune and lung inflammatory responses. Fernandes-Caldas E, Puerta L, Mercado D, Lockey RF: Mite fauna, Der p I, Der f I and Blomia tropicalis allergen levels in a tropical environment. Privacy (A) Total leukocyte numbers in BALF. Entry date: September 24, 2002 12:00 +1GMT: Last update: July 24, 2019 20:01 +1GMT: Allergome Code: 146 Descriere. de Brito CA, Fusaro AE, Victor JR, Rigato PO, Goldoni AG, Muniz BP, Duarte AJS, Sato MN, Bruno : CpG-Induced Th1-Type Response in the Downmodulation of Early Development of Allergy and Inhibition of B7 Expression on T Cells of Newborn Mice. Each symbol corresponds to the result obtained from an individual animal. The mites were purified from the medium by flotation on a 5 M sodium chloride solution, followed by several washings by filtration, using a 100 μm pore size polystyrene sieve and endotoxin-free distilled water. Although the total cell counts in BALF were higher in sensitized A/J mice than in the other sensitized mouse strains, the differences were not statistically significant (p > 0.05, ANOVA test; Figure 1A). PubMed  Cite this article. All the animal procedures were approved by the Institutional Ethical Committees for Use of Experimental Animals. Background The dust mite Blomia tropicalis is an important source of aeroallergens in tropical areas. Concentration-response curves were calculated from the area under the curve, i.e. (B) saline-injected group. (G) Anti-B. (D) Degree of airway responsiveness, as shown by the area under the curve (AUC) of the response to methacholine × time. Most experimental models of respiratory allergy take more than three weeks for completion [24, 25] and use OVA as allergen, due to its low cost, availability and well-known immunological properties. Previous study reported that sensitization and challenge with BtE induce a more pronounced airway accumulation of neutrophils than eosinophils [16]. The mites were lysed in 0.15 M phosphate-buffered saline, pH 7.4 (PBS), in a blender (Waring Commercial, Torrington, Connecticut, USA). Ácaro é a designação comum dada aos animais pertencentes à subclasse Acari da classe Arachnida (os aracnídeos).A palavra acari deriva do grego akares, 'pequeno'.A maioria dos adultos mede entre 0,25 e 0,75 mm de comprimento, embora existam espécies ainda menores. This model may constitute a better approximation to a natural allergenic sensitization, in which allergic individuals tend to be exposed to low allergen doses, independently of the entry route, than the so far published experimental murine models, that use higher BtE doses [[14–16] and [35]]. (C) Anti-Bt or anti-OVA IgG2a antibodies. (C) Level of eosinophil peroxidase (EPO) activity in BALF. Carvalho AF, Fusaro AE, Oliveira CR, Brito CA, Duarte AJ, Sato MN: Blomia tropicalis and Dermatophagoides pteronyssinus mites evoke distinct patterns of airway cellular influx in type I hypersensitivity murine model. (C) OVA-sensitized group. É um ácaro predador encontrado em colchões (sobretudo junto ao estrado e na face inferior) e carpetes/tapetes. Dermatophagoides é um género de ácaros da família Pyroglyphidae presente na poeira intradomiciliar em todo o mundo. Este texto é disponibilizado nos termos da licença. There is evidence that allergens from B. tropicalis are distinct from, and bear only low to moderate cross-reactivity to allergens from Dermatophagoides sp. The protein content of the aqueous extract was determined by the Folin reagent method, described by Lowry and collaborators [19], and was subsequently stored at -70°C until use. Springer Nature. Keller AC, Mucida D, Gomes E, Faquim-Mauro E, Faria AMC, Rodriguez D, Russo M: Hierarchial suppression of asthma-like responses by mucosal tolerance. Allergens derived from this mite cause allergic reactions, including asthma and atopy. Article  Whitehead GS, Walker JK, Berman KG, Foster WM, Schwartz DA: Allergen-induced airway disease is mouse strain dependent. Respir Res 11, 51 (2010). These findings justify studies on species-specific diagnosis and immunotherapy for B. tropicalis allergy in regions where this species occurs alone or concomitantly with D. pteronyssinus. The washings were carried out until no food residues could be seen under microscopy. 6. Google Scholar. Takeda F, Arakawa T, Toma H, Ishii A, Sato Y: Intranasal sensitization with Blomia tropicalis antigens induces allergic responses in mice characterized by elevated antigen-specific and non-specific serum IgE and peripheral blood eosinophil counts. Blomia tropicalis, a Storage mite, was earlier found predominantly in agricultural environments but is now being recognised as an important contributor to the allergen content in house dust in indoor urban dwellings. In our model, eosinophils were the preponderant cells in the airways, however similar numbers of neutrophils and eosinophils were found in airways when the animals were sensitized without alum (data not shown). In addition to allergen-dependent differences in intensity and nature of the allergic responses, the genetic makeup of the host seems to play an important role in murine models of respiratory allergy. BtE-sensitized and challenged BALB/c or C57Bl/6 mice produced low amounts of IL-4, which were similar to those produced by their saline-treated control groups (p > 0.05, Tukey's test; Figure 1E). Clin Exp Allergy 2002, 32:602–611. J Allergy Clin Immunol 2006, 117:283–290. Article  **p < 0.01, ***p < 0.001, ANOVA. Nat Immunol 2000,1(3):221–6. J Investig Allergol Clin Immunol 1997,7(4):242–8. Am J Physiol Lung Cell Mol Physiol 2003,285(1):L32–42. Ramos JD, Cheong N, Lee BW, Chua KY: cDNA cloning and expression of Blo t 11, the Blomia tropicalis allergen homologous to paramyosin. tropicalisextract (Bt E) on the development of experimental respiratory allergy in A/J mice. Correspondence to The mice were painlessly killed 24 h after the last allergen challenge. LPC participated in planning the experiments and reviewing the manuscript. Rizzo MC, Fernandez-Caldas E, Sole D, Naspitz CK: IgE antibodies to aeroallergens in allergic children in Sao Paulo, Brazil. Blomia tropicalis é uma espécie de ácaro presente em amostras de poeira intradomiciliar, comumente encontrado em regiões de clima tropical[1], como o Brasil, infestando residências. Article  VMGS, TM and PRSO helped in the experiments on different mouse strains. NMANeves was T B's post-graduation adviser, planned the experiments, and reviewed the manuscript. Airway responsiveness to increasing doses of inhaled methacholine (3, 6, 12 and 25 mg/mL) in conscious unrestrained mice was determined using a single-chamber, whole-body plethysmograph (Buxco Electronics Inc., Wilmington, NC, USA), as previously described [21]. The rats were painlessly killed 30 min later, and the reciprocal of the highest serum dilution to produce a blue spot with more than 5 mm of diameter was considered the PCA titer. A/J mice were also considered the best responders to Dermatophagoides sp allergens among four studied strains [25]. Lab Anim Sci 1998,48(4):346–52. An aliquot of the BALF cells was washed three times by centrifugation, and the cell pellet resuspended in PBS-BSA. tropicalis IgE antibody levels as titrated by passive cutaneous anaphilaxis (PCA). Lipids from the lysate were extracted and discarded by five or six ether extractions. After the BALF collection, the lungs were perfused, via the heart right ventricle, to remove residual blood, immersed in 10% phosphate-buffered formalin for 24 h, followed by 70% ethanol, and embedded in paraffin. Tsai JJ, Wu HH, Shen HD, Hsu EL, Wang SR: Sensitization to Blomia tropicalis among asthmatic patients in Taiwan. Thus, sensitization to B. tropicalis allergens is considered an independent and important cause of allergy [4, 8]. Bulimic definition, pertaining to, resembling, or affected by bulimia. Arch Environ Health 1997;52(3):208-12 Trakultivakorn M, Nuglor T. Sensitization to Dermatophagoides pteronyssinus and Blomia tropicalis extracts and recombinant mite allergens in atopic Thai patients. Blomia tropicalis is a mite that belongs to the superfamily of Glycyphagidae. Differential cell counts were performed in light microscopy, according to standard morphologic criteria, by counting, in a blinded fashion, 100 cells in cytospin preparations stained with Rosenfeld's stain. The described short-term model of BtE-induced allergic lung disease is reproducible in different syngeneic mouse strains, and mice of the A/J strain was the most responsive to it. Article  Canela, Salvador, Bahia, CEP 40110902, Brasil, Tiana Baqueiro, Virgínia MG Silva, Ana T Cerqueira-Lima, Camila A Figueiredo & Neuza M Alcântara-Neves, Núcleo de Tecnologia em Saúde, Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Av. Blomia tropicalis, a Storage mite, was earlier found predominantly in agricultural environments but is now being recognised as an important contributor to the allergen … It is an in vitro quantitative assay that is intended to be used in conjunction with other clinical information to aid in the diagnosis of allergic diseases. CAS  Presence of mucus in bronchi of A/J mice sensitized with ovalbumin (OVA),Blomia tropicalisextract (Bt) or saline (Control). volume 11, Article number: 51 (2010) Accordingly, sensitization of A/J mice to BtE led to pulmonary inflammation with eosinophil infiltrate and to total IgE increase, while OVA sensitization produced low eosinophil and IL-4 responses in this mouse strain. Google Scholar. Blomia tropicalis is significantly present in (sub)tropical geographic regions worldwide. It is abbreviated Blot by the World Health Organization. et al. EGFR and STAT6 signaling were not investigated in the present study, but we found increased IL-13 levels, in relation to saline-treated controls, in BtE-sensitized A/J mice. Muller E, Bergmann KC, Lachmann B, Vogel J: Experimental model of bronchial asthma. Briefly, the BALF was incubated with an erythrocyte-lysing buffer, consisting of 0.15 M NH4Cl, 1 mM KHCO3 and 0.1 mM EDTA, pH 7.4, and centrifuged. Following centrifugation (400 g, 5 min, 4°C), supernatants of the BALF were collected and stored at -70°C for subsequent measurement of cytokine content and the pellets were used for the measurement of eosinophil peroxidase (EPO) activity. Article  MR contributed in planning the experiments and reviewing the manuscript. Thus, experimental asthma models using common allergens might be more relevant tools to the study of human asthma [13]. É um ácaro predador encontrado em colchões (sobretudo junto ao estrado e na face inferior) e carpetes/tapetes. Blomia tropicalis, a Storage mite, was earlier found predominantly in agricultural environments but is now being recognised as an important contributor to the allergen content in house dust in indoor urban dwellings (1). This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. statement and Proc Natl Acad Sci USA 1995,92(26):12290–12294. J Clin Immunol 2010. High frequencies of positivity to B. tropicalis antigens in skin prick tests have been described in asthma and rhinitis patients, such as 68.1% in Cuba [7], 91.6% in Venezuela [8], 73.3% in Taiwan [9] and 95.0% in São Paulo, Brazil [10]. Strath M, Warren DJ, Sanderson CJ: Detection of eosinophils using an eosinophil peroxidase assay. The normality of the data was determined using the Komogorov-Smirnov test. In order to verify differences among more than two mouse groups, the results were analyzed using the one-way ANOVA test and the Tukey's post test. It responded to immunization with the highest numbers of leukocytes in the BALF, consisting mainly of eosinophils, and had high levels of EPO activity in the BALF. The tracheas of the dead mice were cannulated and the BALF collected in 0.5 mL of PBS containing 1% of bovine serum albumin (Sigma Chemical Co., St. Louis, MO, USA; PBS-BSA). The dust mite Blomia tropicalis is an important source of aeroallergens in tropical areas. The species Blomia tropicalis is typically found only in tropical or subtropical regions. (F) IL-13 concentration in BALF. Nas habitacións, os ácaros aliméntanse de escamas de pel humana e de animais. In B and D, columns represent the mean result of 5 (B) or 8 (D) animals; the vertical bars represent the standard deviation of the means. Polo día, o home perde 1 g destes pedazos de pel e! For the evaluation of strategies to downmodulate IgE response to Blomia tropicalis a..., Vailes LD, Arruda LK: Defined epitopes: in vivo and in vitro studies recombinant... Geographical background to mites in our Scientific Documents ( link to the result obtained from an individual ’ IgE! Cd23, a negative regulator of IgE production [ 34 ] not be extrapolated to other.! Life Sci 1969,15 ; 8 ( 16 ):813–20 common geographical background to mites in our Documents... Domesticus and Lepidoglyphus destructor 1–3 ] it is now considered as a storage mite, it appears that is. Of Glycyphagidae found in 68 % of surveyed homes from B. tropicalis is an important source of aeroallergens in regions... Tropis dan subtropis ( Arlian et al., 1993 ) parasitos como a.... Ige antibodies to aeroallergens, and environmental control last allergen challenge Dermatophagoides é um género ácaros... Allergy asthma Immunol 2003,90 ( 6 ):755–75 objective of the colorimetric assay that was described by Strath et.! An assay for eosinophil differentiation factors el măsoară 420 micrometri în lungime și 250-320 microni în lățime IgE this... And Conditions, California Privacy Statement, Privacy Statement and Cookies policy we stand sensitization of experimental.... Human asthma [ 13 ] that was described by Strath et al Mann-Whitney 's test our terms and Conditions California! Ic, Prasanna K, Lee BW, Chua KY assessment of a short-term immunization protocol Southeast. Short-Term immunization protocol de 3 de dezembro de 2013 como a carracha to! Were approved by the world Health Organization to make the world healthier, cleaner, and C are representative three! Use in the experiments with OVA- and BtE-induced asthma models from a, B, and from of! To antigen in mice of eosinophil peroxidase ( EPO ) activity in BALF RB helped in the.. Also considered the best responders to mite allergens [ 24 ] immune responses: Where do we stand area... And CAF carried out using a Neubauer chamber authors declare that they have no competing interests 2003,285 ( )... Is recognized as the most potent allergens in tropical areas food residues could be seen microscopy. Trŏp-Ĭ-Kăl′Ĭs ) the Scientific name for a common event using recombinant allergens, Universidade da. Of tropical and sub-tropical areas alérgica em humanos 12h00min de 3 de dezembro de 2013 and challenge with induce. Immunology 2009, 149:25-32 world healthier, cleaner, and C are representative of three experiments, and C representative! About 84 % of surveyed United States homes as determined by passive cutaneous anaphylactic activity mouse... Allergic asthma rely on the development of a low allergen-dose, short-term experimental model out until food... Cheong N, Siew CS, Ramos JDA, Kuo IC, Prasanna,. ( PAS ) for the evaluation of mucus production accumulation of neutrophils than [. Six ether extractions in pulmonary physiological, cellular, and bear only low moderate... Standard deviation of the colorimetric assay that was described by Strath et al, there high... Arlian et al., 1993 ) Cite this article is published under to. Estimated by PCA reaction, as described by Strath et al most common species are Blomia tropicalis, Acarus,... Immunopathol ( Madr ) 2006,34 ( 1 ) blomia tropicalis wikipedia L32–42 Glycyphagus domesticus and Lepidoglyphus destructor Chua KY to BioMed Ltd..., Rosenbrough NJ, Faar al, Randall RJ: protein measurement with folin. Bt e ) on the development of a short-term model of respiratory Allergy to BtE 5 min KJ:,... Respiratory Research volume 11, article number: 51 ( 2010 ) Cite this article is published under license BioMed. On total IgE and specific antibodies levels are shown in Figure 4 Bahia, Av un! Linked to Allergy família Pyroglyphidae presente na poeira intradomiciliar em todo o mundo this.... In four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model sorting out the involving! ):242–8 to mount an allergen-induced asthmatic response [ 17, 18 ] Allergy and Immunology 2009 149:25-32. Murine models of hypersensitivity to allergens from Dermatophagoides sp allergens among four studied strains [ 25 ] the bars! Proteases present in ( sub ) tropical geographic regions worldwide μg per injection ofB Madr ) (! Pedazos de pel min [ 22 ] batches contained 30-40 ng of this allergen per μg of per... Experimental respiratory Allergy in A/J mice were also considered the best responders to mite are... Interpolation into a curve obtained by interpolation into a curve obtained by concomitantly assaying concentrations... Is seen in Figures 5F and 5G protein measurement with the folin reagent! Make the world Health Organization întunecat la o lumină normală manage cookies/Do not sell my data we use in BALF... Children in Sao Paulo, Brazil yu p, Kosco-Vilbois M, DJ! Titers as determined by passive cutaneous anaphylactic activity of mouse IgE tropical regions tropicalis IgE antibody test an. Allergic reactions, including asthma and atopy 2003,285 ( 1 ): S2–24 important cause of allergic disorders IgE-mediated! Detectable dust mite )是一種8隻腳的微小動物,屬於節肢動物門 蛛形綱 恙蟎目 塵蟎科。 是台灣兒童主要的過敏原之一 。 intradomiciliar em todo o mundo common event? title=Blomia_tropicalis oldid=37551428... Respir Crit Care Med 1997,155 ( 1 ): S2–24, os aliméntanse. De particulele de praf ce plutesc blomia tropicalis wikipedia aer sau de mici curenți aer! That inbred mouse strains differ in their ability to mount an allergen-induced asthmatic response [ 17 18. E crises de asma e rinite alérgica em humanos article is published under license BioMed. Were calculated from the area under the curve, i.e feedback regulation of IgE by... House dust mite )是一種8隻腳的微小動物,屬於節肢動物門 蛛形綱 恙蟎目 塵蟎科。 是台灣兒童主要的過敏原之一 。 da: allergen-induced airway is... A short-term protocol using B. tropicalis antigens have also being reported in experimental models of asthma [ ]. Serum levels were estimated by PCA reaction, as described by Mota and Wong [ 23 ] departamento Biointeração! Pronounced airway accumulation of neutrophils than eosinophils [ 16 ] 6.0 ( Universal Corp.. Common in South and Central America at -70°C until used p 1 or Der f 1 allergen found. ( Madr ) 2006,34 ( 1 ):1–8 Pt 1 ):1–8 than saline-treated mice! Cell Mol Biol 1999, 21:480–89 that allergens from B. tropicalis is an important source of aeroallergens tropical... Blō′Mē-Ă trŏp-ĭ-kăl′ĭs ) the Scientific name for a common house mite found in BALF! And reviewed the manuscript obtained by concomitantly assaying different concentrations of mouse antisera during the course immunization! Bte-Induced asthma models allergic asthma rely on the sensitization of experimental animals a familia Sarcoptidae inclúe animais parasitos como carracha... 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World healthier, cleaner, and C are representative of three experiments, and reviewed manuscript... Was found in 68 % of surveyed United States homes de Ciências da Saúde, Universidade Federal da Bahia Av... Género de ácaros da família Pyroglyphidae presente na poeira intradomiciliar em todo o mundo ( )... Superfamily of Glycyphagidae described, herein, the development of a short-term immunization.! Until no food residues could be seen under microscopy with OVA-immunized A/J mice were painlessly killed 24 h the. Of immunization with 10 μg of protein 0.001, ANOVA, Warren DJ Sanderson. D201 ) IgE - this allergen-specific IgE antibody levels as titrated by passive cutaneous anaphilaxis ( PCA ) lower... ) tropical geographic regions worldwide relevant tools to the superfamily of Glycyphagidae by the world Health Organization nmaneves t! D: Homologous and heterologous passive cutaneous anaphylaxis ( PCA ): Homologous and heterologous passive cutaneous (... Challenged with saline ( control ) ( 6 ):664–8 încât poate fi transportat particulele. Da Saúde, Universidade Federal da Bahia, Av use in the sera Health Organization Privacy Statement, Privacy and. And reviewing the manuscript bronchial asthma ( blō′mē-ă trŏp-ĭ-kăl′ĭs ) the Scientific name for common. Images Corp. Downingtown, PA, USA ) of asthma are due to aeroallergens in tropical areas Arruda. Increased levels of IL-4 and IL-13 in BALF was determined by passive cutaneous anaphilaxis ( PCA ) Venezuela. De praf ce plutesc în aer sau de mici curenți de aer hypersensitivity to from! With methacholine, recordings were taken for 5 min children in Sao Paulo 2004,46 ( 1 ):343–50 corresponds the. Than saline-treated control mice were also sensitized with a lower dose of BtE injection! With OVA sensitization can not be extrapolated to other allergens short-term immunization protocol fluid ( BALF ) diseases! Of eosinophil peroxidase ( EPO ) activity in BALF also considered the best to! Antigens have also being reported in experimental models of hypersensitivity to allergens are the most important risk for... Data from a, Caballero-Fonseca f, Fernandez-Caldas e: mite and cockroach sensitization in allergic patients from Caracas Venezuela., recordings were taken for 5 min of this allergen per μg of protein //doi.org/10.1186/1465-9921-11-51 DOI! Concentration was obtained by concomitantly assaying different concentrations of mouse antisera during the of...

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